Figure 4
Isolation and structural characterisation of the Hsp90:p53-TMGST complex. (a) Top, size-exclusion profile of the complex (continuous line) compared with the profiles for Hsp90 (dotted line) and p53-TMGST (broken line). Bottom, the selected fractions were analysed by SDS-PAGE and stained with Coomassie Blue. (b) Three orthogonal views of the Hsp90:p53-TMGST complex 3D reconstruction. (c) The same views with docking of Hsp90 (pdb 5fwk; dark blue) and p53-TMGST. The DTSSP crosslinks in the images are described in Supplementary Fig. 6c. Only crosslinks between residues located in regions with known atomic structure are depicted in the figure. Bar, 100 Å for (b,c).
