Table 5 The top 20 level-2 GO terms of DEG unigene set and midgut transcriptome unigene sets.

From: Differential gene expression in response to eCry3.1Ab ingestion in an unselected and eCry3.1Ab-selected western corn rootworm (Diabrotica virgifera virgifera LeConte) population

 

DEG Unigene set

Midgut Unigene Set

Biological Process

GO Terms

Percentage

Rank

Percentage

Rank

metabolic process

29.52%

1

8.56%

1

single-organism process

15.05%

2

3.78%

3

cellular process

13.60%

3

8.09%

2

localization

7.09%

4

1.58%

6

biological regulation

2.75%

5

1.98%

4

response to stimulus

2.17%

6

1.32%

7

regulation of biological process

2.03%

7

1.79%

5

multicellular organismal process

1.45%

8

0.54%

10

developmental process

1.16%

9

0.47%

11

cellular component organization or biogenesis

0.87%

10

1.01%

8

signaling

0.87%

10

0.92%

9

negative regulation of biological process

0.72%

12

0.21%

13

positive regulation of biological process

0.58%

13

0.21%

12

immune system process

0.58%

13

0.11%

18

multi-organism process

0.43%

15

0.14%

16

detoxification

0.29%

16

0.03%

22

cell killing

0.29%

16

0.01%

24

reproductive process

0.14%

18

0.14%

15

reproduction

0.14%

19

0.14%

14

locomotion

0.14%

19

0.09%

19

Molecular Function

GO Terms

Percentage

Rank

Percentage

Rank

catalytic activity

32.85%

1

8.52%

2

Binding

20.98%

2

8.79%

1

transporter activity

3.62%

3

0.83%

3

molecular transducer activity

0.72%

4

0.27%

6

molecular function regulator

0.43%

5

0.23%

7

structural molecule activity

0.43%

5

0.82%

4

electron carrier activity

0.43%

5

0.08%

9

signal transducer activity

0.29%

8

0.30%

5

antioxidant activity

0.29%

8

0.05%

10

Cell Compartment

GO Terms

Percentage

Rank

Percentage

Rank

membrane

9.12%

1

3.64%

3

cell

5.93%

2

4.14%

1

cell part

5.79%

3

4.09%

2

extracellular region

5.35%

4

0.25%

9

membrane part

5.07%

5

2.43%

5

organelle

4.05%

6

2.69%

4

extracellular region part

2.46%

7

0.09%

11

organelle part

1.88%

8

1.43%

7

macromolecular complex

0.87%

9

1.74%

6

supramolecular complex

0.43%

10

0.10%

10

membrane-enclosed lumen

0.43%

10

0.37%

8

  1. The DEGs are from comparison of midgut gene expression between select and unselected-WCR when both were given eCry3.1Ab-expressing roots for 24 hours. The GO terms are ranked by number of sequences of each GO, as well as the percentage of sequences among each unigene set.