Table 3 Matrix effect and recovery of decitabine from enzyme digestion matrix (n = 5).

[Decitabine] (nM)				[dC] (nM)			[5mdC] (nM)			[dG] (nM)
	3.00 (LQC)	70.0 (MQC)	1.60 × 10³ (HQC)	3.00 (LQC)	70.0 (MQC)	1.60 × 10³ (HQC)	3.00 (LQC)	70.0 (MQC)	1.60 × 10³ (HQC)	3.00 (LQC)	70.0 (MQC)	1.60 × 10³ (HQC)
MF_Ana^a ± SD	0.959 ± 0.001	0.972 ± 0.001	1.01 ± 0.00	0.964 ± 0.002	0.976 ± 0.002	1.01 ± 0.03	0.933 ± 0.003	0.951 ± 0.006	0.968 ± 0.003	0.943 ± 0.003	0.961 ± 0.007	0.990 ± 0.002
MF_IS^b ± SD	0.977 ± 0.002	0.981 ± 0.001	0.98 ± 0.01	0.977 ± 0.002	0.981 ± 0.001	0.98 ± 0.01	0.977 ± 0.002	0.981 ± 0.001	0.98 ± 0.01	0.977 ± 0.002	0.981 ± 0.001	0.98 ± 0.01
IS Normalized MF^c ± SD	0.982 ± 0.002	0.991 ± 0.001	1.03 ± 0.01	0.987 ± 0.003	0.995 ± 0.002	1.03 ± 0.03	0.955 ± 0.004	0.970 ± 0.006	0.988 ± 0.01	0.965 ± 0.004	0.980 ± 0.007	1.01 ± 0.01
R_Ana^d ± SD (%)	91.2 ± 0.2	97 ± 1	100 ± 1	92.6 ± 0.3	98 ± 2	100 ± 2	90.3 ± 0.4	95. ± 3	98 ± 1	90.7 ± 0.5	96 ± 4	99 ± 1
R_IS^e ± SD (%)	91 ± 2	99 ± 3	99 ± 2	91 ± 2	99 ± 3	99 ± 2	91 ± 2	99 ± 3	99 ± 2	91 ± 2	99 ± 3	99 ± 2
IS Normalized R^f ± SD	1.00 ± 0.02	0.98 ± 0.03	1.01 ± 0.02	1.02 ± 0.0	0.99 ± 0.04	1.01 ± 0.03	0.99 ± 0.02	0.96 ± 0.04	0.99 ± 0.02	1.00 ± 0.02	0.97 ± 0.05	1.00 ± 0.02

^aMF_Ana = (mean peak area of analyte in extracted enzyme digestion matrix)/(mean peak area of analyte in the mobile phase).
^bMF_IS = (mean peak area of IS in extracted enzyme digestion matrix)/(mean peak area of IS in the mobile phase).
^cIS Normalized MF = MF_Ana/MF_IS.
^dR_Ana = [(mean peak area of analyte in enzyme digestion matrix)/(mean peak area of analyte in extracted enzyme digestion matrix)] × 100%.
^eR_IS = [(mean peak area of IS in enzyme digestion matrix)/(mean peak area of IS in extracted enzyme digestion matrix)] × 100%.
^fIS Normalized R = (R_Ana/R_IS) × 100%.
Note: IS 2′-Deoxycytidine ¹⁵N₃ added at a concentration of 200 nM.

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