Figure 5
From: Skeletonema marinoi as a new genetic model for marine chain-forming diatoms
Schematic representation of the genomic insertion site in selected S. marinoi transformants. Shown on the left are the predicted gene models disrupted by the insertion of the transformed DNA construct (blue triangle) in ten transformants (SM1–2, SM4, SM7, SM10–13 and SM15–16), with the green regions representing exons. The shown genomic regions correspond to at least one mRNA identified by RNA sequencing, the 5′ end of which is indicated by the black arrow. Underneath each gene map is shown a scale corresponding to 500 bp. Also indicated for each transformant are the primers specific for regions flanking the inserted construct used to determine the segregation state as shown on the right, confirming either heterozygosity or homozygosity for the mutation. PCR was performed on genomic DNA extracted from wild type S. marinoi and the indicated transformant. The amplified products were separated and visualized by gel electrophoresis. The identity of all PCR products was confirmed by DNA sequencing, with non-specific products denoted with an asterisk.
