Figure 5

Assessment of unspecific behavioural effects of CNO in the 5-CSRTT. (a) Attentional accuracy (%); (b) relative number of omitted responses (%); (c) relative number of premature responses (%); (d) latency to collect the reward after correct responses (s); (e) latency to make a correct response after the onset of the stimulus (s); (f) number of beam breaks caused by movement between the 5-choice wall and the receptacle; (g) number of perseverative (repeated) responses into the same correct hole; and (h) total number of trials conducted by the animal within a 30-min session during the 5-CSRTT started 10 min after i.p. injection of either vehicle (Veh, black), 3.5 (pink) or 10 mg/kg CNO (purple), as indicated. (i) Plasma concentrations of CNO (nM), determined 45 min after i.p. injection; statistical differences not indicated. *p < 0.05, Dunnett’s post hoc test comparing the value under 3.5 or 10 mg/kg CNO to the vehicle value after significant one-way ANOVA across all three groups. Note that experiments using 3.5 vs 10 mg/kg were conducted separately, each following a between-subject design; data from both vehicle groups were combined. The starting dose was counter-balanced across animals, in each case. Results are shown as data from individual animals (dots) and as population mean ± s.e.m. (horizontal lines). 5-CSRTT, five-choice serial-reaction time task; ANOVA, analysis of variance; CNO, clozapine-N-oxide; i.p., intraperitoneal; s.e.m., standard error of the mean.