Figure 1

Schematic illustration of the experimental procedure. WT and APP/PS1 animals were randomly assigned to four groups (A) Ctrl, (B) SD, (C) JuA and (D) JuA + SD. Group C and D animals were given ICV injection of JuA (0.02 mg/kg) for five consecutive days starting two days before the SD procedure. Groups (B,D) animals were subjected to 72 h SD in individual automated treadmill cages. A set of mice from all groups was sacrificed for patch clamp recordings. Separate sets of animals were further subjected to either a spatial memory probe trial in the Morris water maze (MWM), or tissue collection for western blotting. Mice used for western blotting or electrophysiological experiments did not perform the behavioral test.