Figure 8 | Scientific Reports

Figure 8

From: Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation

Figure 8The alt text for this image may have been generated using AI.

Quantification of hydroxyl radical spin adducts generated via LED irradiation of titanium specimens (Ti) immersed in 3% H2O2 or pure water. Analysis was performed using the electron spin resonance spin-trapping technique using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin trap agent. The yield of DMPO-OH, a spin-adduct of hydroxyl radicals, (a) after treatment for 15 s and (b) 5 min. Irradiation with 365 nm LED of titanium specimens immersed in 3% H2O2 [H(+)L(+)] for 15 s generated 81.6 µM DMPO-OH, whereas other treatments generated only trace levels (<0.2 µM DMPO-OH). Values and error bars indicate the mean and standard deviation, respectively (n = 3). Different letters above the columns refer to significant differences (p < 0.01) between different groups. H(−)L(−), treatment with pure water in a light-shielding box; H(+)L(−), treatment with 3% H2O2 in a light-shielding box; H(−)L(+), 365-nm LED irradiation of sample in pure water; H(+)L(+), 365-nm LED irradiation of sample in 3% H2O2.

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