Table 2 BCR sequencing data summary for biliary remnants and lymph nodes.

From: Oligoclonal immunoglobulin repertoire in biliary remnants of biliary atresia

Biliary remnants

Reads

Distinct CDR3s

D50

Frequency top 100 CDR3s

iBA1

966609

2349

0.1

97.9%

iBA2

712470

6293

0.2

93.5%

iBA3

504036

3189

0.3

95.8%

iBA4

808102

4997

0.2

94.4%

iBA6

113811

1277

0.5

96.5%

iBA8

567690

12631

0.9

53.8%

iBA9

538696

12891

0.9

51.3%

iBA11

224469

2327

0.4

95.8%

BASM12

127299

2089

0.5

95.0%

BASM14

584857

9361

0.6

69.3%

BASM15

584219

7365

0.4

87.1%

BASM16

497679

7917

0.6

76.3%

Lymph nodes

Reads

Distinct CDR3s

D50

Frequency top 100 CDR3s

iBA LN6

443254

36954

23.0

5.7%

iBA LN7

369392

32088

20.2

6.8%

iBA LN9

339861

13263

1.8

37.0%

iBA LN10

343714

25247

12.2

17.7%

iBA LN11

856998

77852

16.3

9.1%

BASM LN12

422420

18560

4.0

22.8%

BASM LN13

423820

30205

11.9

15.3%

BASM LN14

366526

32962

15.0

10.5%

BASM LN15

378908

92206

8.0

15.0%

BASM LN16

458374

26120

8.4

16.4%

  1. One biliary remnant (iBA5) was excluded due to insufficient tissue and three biliary remnants (iBA7, iBA10, BASM13) were excluded due to poor BCR sequencing quality. This yielded a total of 8 iBA biliary remnants, 4 BASM biliary remnants, 5 iBA lymph nodes (3 with paired biliary remnants), and 5 BASM lymph nodes (4 with paired biliary remnants).