Table 1 Summary of results obtained from multiple diagnostic methods for PPRV genetic material or antigen with ocular swabs or fecal material.

From: Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control

 

Sample code

Ocular swabs

Feces

PCR

QPCR

LFD antigen test

PCR

QPCR

AgELISA

Goat

G4

+

no Ct

+

+

26.9

+(68%)

G10

+

22.96

+

+

22.92

+(120%)

G11

+

36.96

+

−

35.64

−(<20%)

G16

n.d.

26.37

n.d.

−

26.9

−(<20%)

G74§

−

no Ct

n.d.

n.d.

no Ct

−(<20%)

Sheep

S14

+

no Ct

+

n.d.

no Ct

−(<20%)

S19

n.d.

no Ct

n.d.

n.d.

no Ct

−(<20%)

S20

n.d.

no Ct

n.d.

n.d.

no Ct

−(<20%)

S31

n.d.

no Ct

−

−

no Ct

−(<20%)

S33

−

no Ct

n.d.

n.d.

no Ct

−(<20%)

S37*

n.d.

no Ct

n.d.

n.d.

no Ct

−(<20%)

Total

11

6

11

5

5

11

11

Total positive

 

4

3

4

2

4

2

  1. Results for reverse transcription Polymerase Chain Reaction (PCR) and lateral flow device (LFD) antigen test are indicated as positive (+) or negative (−) status. Results for quantitative reverse transcription Polymerase Chain Reaction (QPCR) are presented in Ct values, with lower Ct values indicating presence of higher amount of target PPR genetic material and “no Ct” indicating no detection of PPR genetic material. Results of the antigen capture ELISA (AgELISA) are presented as positive (+) or negative (−) status, with OD values converted to Sample/Positive Control % following the manufacturers’ instructions between brackets. Tissue from oral lesions (*) or post-mortem samples (§) were collected instead of ocular swabs in some occasions; n.d., analysis not done.
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