Table 9 Luciferase assay.

From: Effects and possible mechanism of Ruyiping formula application to breast cancer based on network prediction

Cell Line

Signaling pathway

Agonist

Agonist con (ng/mL)

Agonist Incubate time(h)

293 with AP-1

 

TPA

25

6

293 with GAS

stat 1

IFNγ

 

12

293 with ISRE

stat 1/2

Interferon α(IFNα)

 

6

293 with NFκB

NFκB

TNFα

25

6

293 with stat-3

Stat 3

Il-6

10

12

293 with TGFβ

Smad

TGFβ

2.5

6

293 with Erα

 

Erα

 

12

293 with Erβ

 

Erβ

 

12

  1. ISRE: Interferon-Sensitive Response Element.
  2. STAT-3:Signal transducer and activator of transcription 3.
  3. TPA:12-O-tetradecanoylphorbol-13-acetate.
  4. TNFα: Tumor Necrosis Factor α.
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