Figure 3
From: Effect of Spheroidal Age on Sorafenib Diffusivity and Toxicity in a 3D HepG2 Spheroid Model
(a) Hematoxylin and eosin stained thin sections of early-stage spheroids at day 3 with loosely connected cell clusters (black arrows), mid-stage spheroids at day 6 with smooth spheroid surfaces and late-stage HepG2 spheroids starting at day 12 post-seeding with disintegrated spheroid surfaces (arrow). (b) Transmission electron microscopy micrographs of early-stage spheroids at day 3 with extracellular space between plasma membranes (black arrows), mid-stage spheroids at day 6 with tight junctions (black arrows) and late-stage HepG2 spheroids starting at day 12 post-seeding with blebbing of the cell surface (arrowhead), condensed chromatin (arrow) and apoptotic bodies (AB). Scale bar, 100 µm (top panel) and 1 µm (bottom panel).
