Figure 5
From: A novel assay for detection and quantification of C-mannosyl tryptophan in normal or diabetic mice
(a) The levels of C-Man-Trp in selected tissues of diabetic model mice. The C-Man-Trp concentration was measured in the samples of tissues from control (C57BL/6) (n = 5) and KK-Ay (n = 7) mice at 16 weeks as described in Materials and Methods. Data represent the mean ± SD. **P < 0.01 indicates a significant difference versus C57BL/6. NS, not significant. (b) Transcription levels of DPY-19 gene homologues in brain (upper), liver (middle), and kidney (lower), of diabetic model mice. The transcripts of DPY19L1-L4 were amplified by RT-PCR and analyzed by agarose gel electrophoresis for the tissues from control (C57BL/6 mice, 16 weeks) and KK-Ay (16 weeks) mice as described in Materials and Methods. Band intensity was quantified as described in Materials and Methods. Data represent the mean ± SD. *P < 0.05 indicates a significant difference versus C57BL/6. NS, not significant.
