Figure 1

Natural colonization and oviposition behavior of Nematus oligospilus on Salix leaves. (a) Feeding damage in the field (Number of plants damaged). Mann Whitney U test P < 0.0001, N = 48 for S nigra and N = 50 for S. viminalis (b) Time spent by females on the leaf surface of S. nigra and S. viminalis during behavioral observations. Mann Whitney U test, NS, N = 12. (c) Scheme showing the three main behaviors performed by females on willow leaves (see description in Table 1). (d) Relative frequency of behavioral sequences (%) displayed by females once on the leaf surface of S. nigra (N = 68 sequences, 12 females) or S. viminalis (N = 59 sequences, 12 females). Chi square between S. nigra and S. viminalis was performed for each sequence showing significant differences for AW-out (p = 0.02), AW-BA (p = 0.03) and AW-EG-AR (p < 0.001) and non-significant differences for AW-others (p < 0.05).