Figure 3 | Scientific Reports

Figure 3

From: Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function

Figure 3The alternative text for this image may have been generated using AI.

(a) Amplitude map of Objx, and Objy, amplitude parameter β, phase contrast for Objx and Objy, phase difference Δϕ relative to a control sperm cell (0 h in heparin). Colorbars indicate the mapping of the phase variation. The Δϕ map shows a birefringence distribution (bright pattern) over all the sperm head. (b) The same holographic parameters measured for a reacted sample (4 h in heparin). The Δϕ map after 4 h in heparin shows a clear decrease of the birefringent area. In particular, the birefringence (bright pattern) is confined in the post-acrosomal region. (c) Distribution of birefringence patterns of sperm from 3 donors exposed to heparin for 0 h (control sample), and 4 h (reacted sample). Scale bar: 4 μm.

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