Figure 4
From: New Therapeutic Approach for Targeting Hippo Signalling Pathway
Intracellular localization of FITC-labelled NLS18 and NLS23. (a) MDA-MB-231 cells (3 × 104 cells/ml) were grown in Labtek chamber slides cover slips and incubated 4 h with 30 μM of FITC-labelled peptides. Cells were washed 3 times with PBS, fixed with 4% paraformaldeyde (PFA) and analyzed by fluorescence microscopy. (b) Time-dependent nuclear localization of FITC-labelled NLS18 and NLS23. MDA-MB-231 cells (3 × 104 cells/ml) were grown as above and incubated with 30 μM of peptide for different periods of time. Cells were washed 3 times with PBS, fixed with 4% PFA and analyzed by fluorescence microscopy.
