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Figure 1

From: Pore-forming protein complexes from Pleurotus mushrooms kill western corn rootworm and Colorado potato beetle through targeting membrane ceramide phosphoethanolamine

Figure 1

Specificity of aegerolysins for binding to multilamellar lipid vesicles with various lipid compositions. The aegerolysins were incubated with multilamellar vesicles and centrifuged as described in the Methods. The supernatant (S) and pellet (P) fractions were subjected to SDS-PAGE and stained with SimplyBlue SafeStain. (A) Binding of OlyA6, PlyA2, EryA, and Cry34Ab1 to vesicles composed of equimolar lipid mixtures, as indicated. (B) Binding of OlyA6, PlyA2, and EryA to vesicles composed of CPE, POPC, and cholesterol (molar ratio, 5/47.5/47.5). Control experiments without the vesicles showed no sedimentation of the aegerolysins. CPE, ceramide phosphoethanolamine; Chol, cholesterol; SM, sphingomyelin; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine.

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