Figure 4 | Scientific Reports

Figure 4

From: Pore-forming protein complexes from Pleurotus mushrooms kill western corn rootworm and Colorado potato beetle through targeting membrane ceramide phosphoethanolamine

Figure 4

Permeabilization of small unilamellar vesicles composed of CPE, POPC, and cholesterol (molar ratio, 5/47.5/47.5) by OlyA6/PlyB, PlyA2/PlyB, and EryA/PlyB. Fluorescence intensity of calcein released from the lipid vesicles, monitored as described in the Methods. (A) Permeabilization of lipid vesicles by 10 μg/mL aegerolysin with PlyB (molar ratio, 12.5/1). (B) Concentration dependence of lipid vesicle permeabilization for OlyA6/PlyB, PlyA2/PlyB, and EryA/PlyB. Data are means ± standard error from three repetitions.

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