Figure 3
Fluctuation units (χ) (equation (6)) of many melanosomes in the case of [ciliobrevin] = 0 μM. (a) The calculations in (b–d) for a single melanosome were repeated for 62 different melanosomes from two different melanophore preparations, and χ was obtained as a function of Δt for 62 melanosomes. Each colour represents a group classified by the clustering method depicted in (b). (b) Cluster analysis by affinity propagation in the case q = 0.3, where q is the sole parameter of the affinity propagation analysis. χ as a function of Δt had four clusters in the case q = 0.3. (c) Number of clusters obtained by affinity propagation as a function of q. Cluster number 4 was the most stable. (d) Histogram of χ* (equation (10)) obtained from the values of χ in (a). There are four FPUs for melanosome transport. (inset) The population (Ni, where i = 1, 2, 3, 4) of each FPU was calculated.
