Figure 4

Human fetal femur-derived skeletal cells were cultured as a three-dimensional pellet over 21 days in chondrogenic media to generate cartilage tissue. (a) Raman spectrum in the CH-stretch region. The three marked bands were targeted for CARS imaging. (b) SHG images fibres of collagen (green) in the bioengineered cartilage tissue. (c) CARS images of the bioengineered cartilage tissue at the CH-stretch region: 2845 cm⁻¹ (ν_s(CH₂)), 2935 cm⁻¹ (ν_s(CH₃)), and 3030 cm⁻¹ (ν_as(CH₃)). Scale bars correspond to 50 µm.