Figure 5

Human fetal femur-derived skeletal cells were cultured as a three-dimensional pellet over 21 days in chondrogenic media to generate cartilage tissue. (a) Raman spectrum in the ‘fingerprint’ region. The three marked bands were targeted for CARS imaging. (b) CARS images acquired at the ‘fingerprint’ vibrations of: 1061 cm⁻¹ (ν_s(OSO₃⁻)), 1450 cm⁻¹ (δ(CH₂)), and 1668 cm⁻¹ (ν(C=O)). Scale bars correspond to 50 µm.