Figure 3 | Scientific Reports

Figure 3

From: The Clock Mechanism Influences Neurobiology and Adaptations to Heart Failure in Clock∆19/∆19 Mice With Implications for Circadian Medicine

Figure 3

Basal dendrite morphology, baseline and HF. Basal dendrite morphology was analyzed for mPFC layer 2/3 and hippocampus CA1 pyramidal neurons using a modified three-dimensional Sholl analysis. This analysis measured the length of basal dendrite between concentric spheres radiating outward from the soma. (a) Normal Clock∆19/∆19 mice exhibit similar basal dendrite length compared to WT mice in the mPFC (left) and the hippocampus (right). (b) WT HF mice exhibit increased basal dendrite length in the mPFC (left, P = 0.0007), and in the hippocampus CA1neurons (right, P = 0.049), versus WT controls. In contrast, (c) Clock∆19/∆19 HF mice exhibit no difference in basal dendrite length in the mPFC (left) and increased basal dendrite length in the hippocampus neurons (right, P = 0.001), versus Clock∆19/∆19 controls. (d) Thus HF is associated with changes to basal dendrite length that differ in magnitude and direction for mPFC neurons, but are similar for hippocampus neurons, between Clock∆19/∆19 HF and WT HF mice. For mPFC: n = 4 mice per baseline group, n = 5 mice per HF group. For hippocampus: n = 4 mice per baseline group, n = 4 mice per HF group. Four neurons were traced and averaged for each mouse, and data are shown as mean ± SEM. *Indicates P < 0.05 by Bonferroni post-hoc analysis.

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