Table 2 Tryptic peptides of bovine RPE65 identified using MS-coupled acyl exchange labeling method.

From: The dual roles of RPE65 S-palmitoylation in membrane association and visual cycle function

Identified peptides of RPE65

Cys Modification

C45GPGLFEVGSEPFYHLFDGQALLHK

NEM

IVITEFGTC106AFPDPC112K

106: NEM

112: NEM; 4-VP #

GVEVTDNALVNIYPVGEDYYAC146TETNFITK

4-VP (NEM # )

QVDLC169NYVSVNGATAHPHIENDGTVYNIGNC195FGK

169: NEM

195: NEM#

SEIVVQFPC231SDR

NEM

KFLSSWSLWGANYMDC278FESNETMGVWLHIADK

n.d

TSPFNLFHHINTYEDHEFLIVDLC329C330WK

329, 330: NEM

NLVTLPNTTATAILC396SDETIWLEPEVLFSGPR

NEM

PYTYAYGLGLNHFVPDRLC448K

NEM

  1. Cysteine residues are underlined, with superscript number showing residue position. Cysteine residues in bold were modified by 4-VP indicating palmitoylation as detected using Synapt G2-Si HDMSE. Modification marked with # in superscript was identified using AB Sciex6600 w/SelexION mass spectrometer. NEM: N-ethyl maleimide; 4-VP: 4-vinyl pyridine.
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