Figure 4

Differential expression of transcripts from RNA-SEQ. (a–d) Volcano plots comparing fold-change and p-value of each identified transcript accession for (a,b) U87 mg and (c,d) DAOY cellular aggregates for samples exposed to 250 V/m dcEFs for (a,c) 2 h, or (b,d) 8 h compared 0 h, unexposed controls. Significance threshold is set to FDR < 0.05 and a Log₂(fold-change) of > 1 or < −1 (n = 3 for all conditions). Differentially expressed transcripts are shown in red. Clustermaps for transcripts per million of differentially expressed transcripts for: (e) U87 mg after 2 h; (f) DAOY after 2 h; (g) U87 mg after 8 h; (h) DAOY after 8 h; relative to 0 h unexposed controls. Each column represents a single replicate sample (U = U87 mg, D = DAOY). Note: for 8 h results, not all differentially expressed transcripts are shown, as Daoy significance cutoff was set to p < 0.0005 and U87 mg to p < 0.005 for improved readability. (i,j) Fold-change of most significantly differentially expressed transcripts. Comparing (i) U87 mg, or (j) DAOY differentially expressed genes for 2 h, or 8 h of dcEF relative to 0 h unexposed controls (U = U87 mg, D = DAOY). Not all differentially expressed transcripts are shown, as Daoy significance cutoff was set to p < 0.001 for improved readability.