Figure 1
From: Convergence of TGFβ and BMP signaling in regulating human bone marrow stromal cell differentiation
Functional heterogeneity of bone- and non-bone- forming hBMSC clones. (a) Quantification of percent ALP activity on day 14. Data is presented as the means ± SD of three independent experiments; n = 6; ***p < 0.0005. The upper image panel shows OsteoImage™ staining, while the lower panel shows Alizarin Red S staining. (b) qRT-PCR quantification of ALPL, RUNX2, OCN, ON, OPN, BMP4, and COL1A1 osteoblast markers under osteoblastic induction conditions. The expression of each target gene was normalized to GAPDH. Data are presented as mean ± SD from three independent experiments, n = 9; *p < 0.05; **p < 0.005, ***p < 0.0005. (c) Nile red quantification of mature adipocytes on day 7 post adipocyte induction of the indicated hBMSC clone. Data are presented as mean ± SD, n = 9 from three independent experiments. ***p < 0.0005. Upper panel shows Nile red staining of mature oil filled adipocytes, while the lower panel shows oil red O staining for adipocyte (20× magnification). (d) qRT-PCR quantification for aP2, LPL and PPARγ2. The expression of each target gene was normalized to GAPDH. Data is presented as the means ± SD from three independent experiments, n = 9; ***p < 0.0005.
