Figure 5 | Scientific Reports

Figure 5

From: Accurate target identification for Mycobacterium tuberculosis endoribonuclease toxins requires expression in their native host

Figure 5

Weak or uncleaved in vivo synthesized tRNAs mirror the subset of weakly cleaved in vitro synthesized tRNAs. Total M. tuberculosis RNA was incubated with (+) or without (−) recombinant VapC-mt11. Northern analysis of (a) tRNAAla31 (b) tRNAThr5 (c) tRNALeu15 (d) tRNASer28 (e) tRNASer24 (f) tRNASer26. Position of the oligonucleotides used are shown by the red dots on the tRNA diagrams above each bracketed group. Cleavage positions, yellow arrow. Oligonucleotides were designed to hybridize to the ASL to optimally differentiate between tRNA species. Therefore, cleavage products are not visible. Hybridization temperatures were optimized to preclude cross-hybridization to other tRNAs. tRNA numbering and anticodon sequences of each numbered tRNA from the Lowe lab genomic tRNA database http://gtrnadb.ucsc.edu33. All uncropped images shown in Supplementary Information Fig. 4.

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