Figure 4
From: Hox gene expression determines cell fate of adult periosteal stem/progenitor cells
Hox-positive periostea contain primitive periosteal stem/progenitor cells. (A–H) Flow cytometry of Hox-negative (frontal and parietal) and Hox-positive (tibial and hyoid) periosteal cells (n = 3). (A,B) Hox-positive skeletal elements contain greater numbers of periosteal stem cells (PSCs), characterized using a gating strategy described by Debnath et al.10. Negative selection of the endothelial (CD31), lymphoid (CD45), erythrocyte (Ter119), and other committed progenitor (Thy, 6C3, and CD105) compartments revealed the subset of cells that were CD200+ PSCs (B) Negative selection of the CD31, CD45, Ter119 compartments revealed the subset of cells that were Sca1+ (D), CD146+ (F), CD166+ (H). The left panels demonstrate the proportion of periosteal stem cells (A), Sca1+ (C), CD146+ (E), and CD166+ (G) cells as a percentage of total periosteal cells. *p < 0.05, ***p < 0.001. Error bars are SEM.
