Figure 5

Resection of DTA-1-containing TDLN reduced antitumor immunity. (a) The attenuation of antitumor effect of DTA-1-treated TDLN resection. DTA-1 Ab was injected into tumors 7 days after tumor inoculation, following TDLN resection 4 hour after Ab injection (n = 6). Tumor volumes were measured at the indicated days. (b) The effect of DTA-1 Ab-injected TDLNs on antitumor effect. CT26 subcutaneous tumors were established on both legs of BALB/c mice. Then, DTA-1 Ab was injected into one side, following TDLN resection of the same side (black circle) or the opposite side (black triangle) (n = 6). P values for volume of DTA-1-injected tumor with removal of TDLNs of injected tumors vs DTA-1-injected tumor with removal of TDLNs of uninjected tumors (*), and volume of DTA-1-uninjected tumor with removal of TDLNs of injected tumors vs DTA-1-uninjected tumor with removal of TDLNs of uninjected tumors (**), as analysed by t-test.