Figure 1
Scheme of the overall experimental set-up. First, the protein content of the secretome of activated PSCs was analysed. On this basis, predictions were made about the functional consequences, which the secreted proteins would have in recipient cells. Second, PT45P1 tumour cells were grown in media conditioned with secretome. The intracellular proteome was studied and again used for functional predictions. Both the predictions from secretome and intracellular proteome were compared and validated by investigating the actual functional variations observed and by identifying relevant regulative factors.
