Figure 6 | Scientific Reports

Figure 6

From: Iterative screening methodology enables isolation of strains with improved properties for a FACS-based screen and increased L-DOPA production

Figure 6

Δhmx1 also improved activity for a second P450 enzyme. The deletion of heme oxygenase HMX1 improves flux through another P450 enzyme, CYP102A1 (BM3). (A) CYP102A1 oxidizes fed indole to indoxyl. Indoxyl spontaneously dimerizes to form indigo, which is dark blue and precipitates out of solution. (B) Strains containing CYP102A1 (BM3) produce indigo when fed indole. The Δhmx1 strain produces approximately 22 +/− 7% (s.e.m) more indigo than the WT strain. Six samples were taken for WT condition and 8 samples were taken for the Δhmx1 condition. Boxes represent the interquartile range (IQR; 25–75%). Whiskers represent the range of the data with the exception of outliers. Data points outside of the IQR +/− 1.5xIQR are considered outliers and identified with diamonds next to the marker. Significance was determined using a t-test.

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