Figure 3 | Scientific Reports

Figure 3

From: Single-cell assessment of transcriptome alterations induced by Scriptaid in early differentiated human haematopoietic progenitors during ex vivo expansion

Figure 3

 BioMark single cell q-RT-PCR of sorted CD90− and CD90+ cells after vehicle control or Scriptaid expansion. (a) Individually plotted relative gene expression levels of single cells (black outline: HSC-associated genes; purple outline: Myeloid-associated genes; blue outline: Lymphoid- and HSC-associated genes and red outline: Erythroid or platelet-associated genes from day 5 Scriptaid-expanded Lin−CD34+CD38−CD45RA−CD90+CD49f+ cells (C1), vehicle-expanded Lin−CD34+CD38−CD45RA−CD90+CD49f+ cells (C2), Scriptaid-expanded Lin−CD34+CD38−CD45RA−CD90−CD49f+ cells (C3), vehicle-expanded Lin−CD34+CD38−CD45RA−CD90−CD49f+ cells (C4). P values were generated using one-way ANOVA with multiple comparisons on Prism 7.0 (*p < 0.05; **p < 0.005; ***p < 0.001). (b) Three-dimensional Principal Component Analysis (PCA) with the variance between individual cells for 34 reference genes and 50 selected differentially expressed (DE) genes plotted. CD90−CD49f+ (C3 and C4) cells can be clearly distinguished from CD90+CD49f+ (C1 and C2) cells from both day 5 vehicle and Scriptaid expanded cell cultures.

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