Figure 5
NPsFA-AS1411/DOX/a221 cooperatively downregulates the miR-221 pathway and modulates P-gp levels in K562DR cells. (A,B) qPCR measuring RNA levels of miR-221, p27kip1 (A) and DNMT1, p15ink4b (B) in K562DR cells treated with the indicated nanoparticles for 48 hours. Data represent three independent experiments and are expressed as the mean ± SD; *P < 0.05, **P < 0.01. (C) Western blot analyzing p27kip1, P-gp and DNMT1 protein expression levels in K562DR cells treated with the indicated nanoparticles for 48 hours. Note: NP, NP-a, NP-A/a, NP-A/D, and NP-A/D/a abbreviations indicate NPsFA, NPsFA-a221, NPsFA-AS1411/a221, NPsFA-AS1411/DOX and NPsFA-AS1411/DOX/a221, respectively.
