Figure 3 | Scientific Reports

Figure 3

From: Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain

Figure 3

Promoters of the IGFBPL1 and the DIP2B genes and their characterization. Promoters of both IGFBPL1 (a) and DIP2B (b) are underlined with broken line for DNase I hypersensitive site according to ENCODE data of UCSC browser. The 5′ 67-bp fragment of the DIP2B consisting of a few CpGs that were bound by CXXC during the affinity purification of genomic fragments. CpG, red); and A, T, and AT stretches, blue. (c) Luciferase activity expressed from pIGFBPL1-312-GLuc (blue) and the empty vector pGLuc Basic 2 (red) in HEK293T cells that were co-transfected with effector plasmids encoding N782 and the domains. The activity of the culture co-transfected with pIGFBPL1-312-GLuc and pEGFP-NLSR is defined as 1.0. Small * shows EGFP. (d) Luciferase activity expressed from pDIP2B-557-Gluc and the empty vector. (e) The same-type experiment as in (c) but co-transfected effector plasmids were free of the EGFP tag sequence. Large * shows a significant difference by t-test (p < 0.05, n = 4).

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