Figure 6

Overexpression of OsEPF1 results in an increase in root cortical aerenchyma. Spatiotemporal formation of aerenchyma in 60-day old plants induced by constitutive overexpression of OsEPF1 and OsEPFL9. (a) RCA was spatiotemporally evaluated in two positions; 2 cm and 13 cm from the roots tips. (b–g) Sections taken at 13 cm (b–d) and at 2 cm (e–g) of WT, OsEPF1OE and OsEPFL9OE roots. (h) Estimated aerenchyma formed in the two root regions. Different letters above the columns indicate significant differences between lines and sampling region (p < 0.05). All values are represented as the mean ± SEM. N = 4–7. Scale bar = 50 µm.