Table 1 Comparative catalytic efficiencies of Casp6 variants.

From: Identification of Allosteric Inhibitors against Active Caspase-6

 

KM (µM)

kcat (s−1)

kcat/KM (s−1 M−1)

Casp6-WT

31.2 ± 6.7

2.27 ± 0.30

76,008 ± 18,626

Casp6-A34E

22.7 ± 0.8

2.04 ± 0.10

89,803 ± 6,453

Casp6-E35K

37.1 ± 4.9

1.71 ± 0.13

46,833 ± 7,641

Casp6-A109T

34.5 ± 1.8

2.51 ± 0.16

72,683 ± 2,529

Casp6-T182S

33.3 ± 1.2

2.42 ± 0.12

72,649 ± 1,192

Casp6-L200A

315.5 ± 162.0

0.05 ± 0.03

150 ± 24

Casp6-K284A

32.6 ± 10.8

2.0 ± 1.5

58,650 ± 30,269

Casp6-WT-LS-SS

24.8 ± 4.4

2.6 ± 0.4

109,148 ± 21,060

Casp6-L200A-LS-SS

196.2 ± 84.4

2.8 ± 0.8

15,155 ± 2,351

Casp6-K284A-LS-SS

27.7 ± 2.7

2.8 ± 0.2

99,882 ± 9,723

  1. ND – not detectable, Data represent mean ± SD of at least three independent experiments.
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