Figure 2
From: The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
EtATG8 of E. tenella sporozoites was lipidated under different conditions. (a) The E. tenella sporozoites incubated in DMEM, HBSS, DMEM with RP (10 μM), HBSS with 3-MA (25 mM) and DMEM with RP (10 μM) and 3-MA (25 mM) at 41 °C for 8 h. Lysated parasites were fractionated in 12% SDS-PAGE without urea or with 6 M urea. WB was labelled with anti-rEtATG8 antibody or anti-Actin antibody. (b) The ratio of EtATG8 form II/form I shown in (a). (c) The ratio of EtATG8 form II/ EtActin shown in (a). The ratio of EtATG8 form II/form I or form II/EtActin under different conditions was presented as mean ± SD of three independent experiments, values with different letters are significantly different at p < 0.05; values with same letters are insignificantly different at p > 0.05 (n = 3).
