Figure 4
Measuring anti-IFN-γ (amino acid 121–131) autoAbs by colorimetric ELISA. (a) The protocol of direct ELISA for measuring anti-IFN-γ amino acids 121–131 autoAbs has been modified by coating with synthetic peptide (IFN-γ amino acid 121–131) and adding 103 dilution of tested serum followed by adding detection Ab (dAb). (b) High production of anti-IFN-γ autoAbs in tested patient serum (P4). Quantitative data (optical density, O.D.) are shown as mean ± SD of three independent experiments. ***p < 0.001, compared with H1.
