Figure 3
Image quality at 10 weeks after the craniotomy implant. Three-dimensional reconstruction from a two-photon fluorescence image of the cortical vasculature. (Ai) Maximum intensity projection (MIP) of cortical vascular architecture in a single rat to a depth of 660 µm below the pial surface. Estimated arrangement of the anatomical layers in the cortex is indicated in roman numerals along the left side (Yusufogullari et al.45). Scale bar: 100 µm. (Aii) Corresponding axial slices at the pial surface, 200 µm, 400 µm, and 600 µm cortical depths. Scale bar: 100 µm. (Aiii) Signal intensity profile across a single blood vessel randomly selected in each axial slice. Pixel intensity was normalized to the brightest pixel in the pial surface slice. Colour line on the axial slice indicates the location of the line on the chosen vessel. (B) The mean and standard deviation of pixel intensity normalized to the brightest pixel on the pial slice for 0 µm, 200 µm, 400 µm, and 600 µm cortical depths. Three vessels parallel to the imaging plane were chosen in each axial slice from all rats acquired at 10 weeks after the craniotomy. The vessels sampled within each slice were averaged and then normalized for comparison between rats.
