Figure 3 | Scientific Reports

Figure 3

From: Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels

Figure 3

Endothelial cell network possess hollow structures when cultured in EGM-2 and SFM. 3D hydrogel constructs after 14 days of cultivation in EGM-2 (a–d) or SFM (e–h) containing GFP positive HUVECs and hASCs after incubation with Texas red-labeled dextran. (a,e) Images of GFP-Fluorescence. (b,f) Images of Texas red fluorescence. (c,g) Overlay of GFP and Texas red. (d,h) Virtual stacks of HUVECs co-cultured with hASCs and incubated with Texas red-labeled Dextran. EGM-2: endothelial growth medium-2, SFM: serum-free medium, Scale bar: (a–c,e–g) 200 µm, (d,h) 30 µm.

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