Table 1 Comparison of network parameters.

From: Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels

 

SFM/Matrigel/rCOL

SFM/hCOL

Significance

Mean Diameter (µm)

9.3 ± 2.4

12.2 ± 3.3

ns

Number of nodes

113.0 ± 19.3

81.7 ± 4.0

ns

Total branching length (µm)

10747.0 ± 938.9

8357.3 ± 526.6

*

Network area (µm2)

(3.2 ± 0.3)*105

(3.2 ± 0.1)*105

ns

Network volume (µm3)

(7.7 ± 1.0)*105

(10.4 ± 0.8)*105

*

Number of segments

207.3 ± 34.6

146.3 ± 4.2

*

  1. Constructs containing GFP-HUVECs and hASCs and Matrigel/rCOL or hCOL were cultivated in SFM. After 14 days of cultivation multiphoton images were taken and processed with Imaris. Data is presented as mean ± standard deviation (SD) for the following parameter: mean diameter, number of nodes, total branching length (sum of the lengths of all segments within the 3D network), network area (the sum of areas of all segments within the 3D network), network volume (the sum of volumes of all segments within the 3D network), and number of segments. Data was analyzed with unpaired t-test. (*) p-value ≤ 0.05, ns: not significant.
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