Figure 5

Neuritogenesis is impaired in Negr1^−/− hippocampal neurons. Confocal images of DIV 0.25 hippocampal neurons derived from P0–1 Wt (a–d) and Negr1^−/− (e–h) mice. Phalloidin staining marks F-actin (red), MAP2 for neurons (green), and DAPI stains nuclei (blue). Scale bar is 10 μm. Graph (i) represents quantification of F-actin intensity for actin aggregates at the neurite initiation site (μm²). Scanning electron microscopy images of Wt (j,l), Negr1^−/− (k,m), hippocampal neurons at DIV0.25. Scale bar in (j,k) is 5 μm, in (l,m) 2 μm. Representative images of pAAV-hSyn-RFP transfected hippocampal neurons derived from Wt (n) and Negr1^−/− (o) mice. Scale bar is 50 μm. Graph (p–r) represents the number, length, and branch points of the neurites per neuron obtained by neuron tracing Neuroleucida. N = 75–82 neurons evaluated per genotype from three independent experiments. Data represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, Mann–Whitney U test (Wilcoxon rank sum test).