Figure 5

Tumour-infiltrating immune cells following CED of cisplatin and/or GL-GM immunizations. Frozen brain sections were stained for CD8⁺, F4/80⁺ and CD206⁺ cells and the percentage of stained area was determined. The median and range of each group are shown. (A) Tumour infiltration of CD8⁺ cells was increased in GL-GM-immunized mice compared with non-treated (*p = 0.0286) and cisplatin-treated mice (*p = 0.0159). (B) Representative staining of CD8 (green) in tumours. (C) Tumour infiltration of F4/80⁺ cells was reduced in GL-GM-immunized mice compared with non-treated mice, both as a monotherapy (non-treated vs. GL-GM, *p = 0.0286) and in combination with cisplatin (non-treated vs. cisplatin + GL-GM, *p = 0.0159). (D) Representative staining of F4/80 (red) in tumours. (E) The majority of F4/80⁺ (red) cells expressed CD206 (green). (B,D,E) Show representative stainings from one animal of each treatment group. Images were taken at 10x magnification. DAPI was used as a nuclear staining.