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Figure 1

From: Novel avian paramyxovirus-based vaccine vectors expressing the Ebola virus glycoprotein elicit mucosal and humoral immune responses in guinea pigs

Figure 1

Multi-step growth kinetics of rNDV-3FHN and rAPMV-3 in embryonated chicken eggs and EBOV GP-expressing recombinants in DF-1 cells. (a) Schematic representation of rNDV-3FHN and rAPMV-3. The genes derived from NDV and APMV-3 are shown as black or gray respectively. (b) Multicycle growth kinetics of rNDV-3FHN and rAPMV-3. 10-day-old embryonated chicken eggs were inoculated with 100 PFU of each virus. Infectious allantoic fluids from eggs were harvested at 12 h intervals until 48 h p.i. and virus titers were determined at each time point. Results of two independent experiments are shown, and error bars show standard deviation. (c) and (d) Schematic representation of GP-expressing rNDV-3FHN and rAPMV-3. The EBOV GP gene was flanked by gene start and gene end signals of NDV or APMV-3 and inserted into indicated intergenic region of those antigenomic cDNA. (e) and (f) Multicycle growth kinetics of rNDV-3FHN and rAPMV-3. DF-1 cells in six-well plates were infected with parental and recombinant viruses at an M.O.I. of 0.01. Supernatants were collected at 8 h intervals until 56 h p.i. and virus titers were determined at each time point. Results of three independent experiments are shown, and error bars show standard deviation.

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