Figure 1 | Scientific Reports

Figure 1

From: Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance

Figure 1

hASCs induced to undergo adipogenesis for 24 hours show a change in the polysome/monosome ratio and have a reduction in proliferative activity. (A) The polysome profile obtained by sucrose density gradient of hASCs treated with control (NI, blue line) or adipogenic induction medium (I, black line) for 24 hours. (B) Quantification of the ratio between monosomal and polysomal areas (n = 3). Mean with SEM; Shapiro-Wilk normality test: NI p = 0.02; I p = 0.90; Mann-Whitney test. (C) Cell proliferation analysis by EdU incorporation assay of hASCs treated with control or adipogenic induction medium for 24 hours (n = 3). Mean with SEM; Student’s unpaired t test analysis: **p < 0.01. (D) Cell cycle analysis of hASCs treated with control or adipogenic induction medium for 24 hours. The cells were stained with propidium iodide and analyzed by flow cytometry (n = 4). Mean with SEM; Student’s unpaired t test analysis: *p < 0.05; ***p < 0.001.

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