Figure 3

Effect of MYOCD siRNA treatment on expression of hypertrophic genes (ANP and β-MHC) and on cardiomyocyte size in RAL rats (n = 8). (a) mRNA expression of MYOCD, ANP and β-MHC in RAL and MYOCD siRNA treated RAL rats. (b) Representative western blot for MYOCD, ANP and GAPDH proteins in RAL and MYOCD siRNA treated RAL rats (before and after ligation). (c) Haematoxylin and Eosin (H & E) stained photomicrographs (10X) of rat hearts: (I) Control group, (II) RAL group (III) Scrambled treated RAL group IV) MYOCD siRNA treated (before ligation) RAL Group V) MYOCD siRNA treated (after ligation) RAL group. Bar diagram shows mean of cardiomyocyte area (n = 50) as measured by Image J analyser. Normalization of total RNA and protein was done using GAPDH as an internal control. Data given is Mean ± SEM; **p < 0.01; ***p < 0.001 for Control vs RAL or scrambled; ^##p < 0.01 for RAL or Scrambled vs RAL treated with MYOCD siRNA before ligation (Pre-ligation); ^$p < 0.05, ^$$p < 0.01 ^$$$p < 0.001 for RAL or Scrambled vs RAL treated with MYOCD siRNA after ligation (Post-Ligation).