Table 1 Final ranking of candidate reference genes from dsRNA-treated beetles according to values given by GeNorm, NormFinder, BestKeeper, and delta Ct and a comprehensive ranking by RefFinder.

From: RNA interference and validation of reference genes for gene expression analyses using qPCR in southern pine beetle, Dendroctonus frontalis

Gene

GeNorm

NormFinder

BestKeeper

delta-CT

Comprehensive

M

R

SV

R

SD

R

SD

R

GM

R

rps18

0.028

1

0.81

7

0.65

1

0.94

5

2.43

1

ef1a

0.028

1

0.773

6

0.67

2

0.92

4

2.63

2

rpl13

0.617

6

0.336

3

1.44

7

1.08

7

5.66

7

ak

0.501

5

0.165

1

1.41

6

0.97

6

3.83

6

sdf

0.1

2

0.666

5

0.71

3

0.87

3

3.41

5

rpl32

0.326

4

0.206

2

1.11

5

0.84

1

2.66

3

tub

0.177

3

0.546

4

0.79

4

0.86

2

3.36

4

ubiq

1.16

7

2.777

8

3.1

8

2.79

8

8

8

  1. M: gene expression stability; R: ranking; SV stability value; SD: standard deviation; GM: Geomean value.
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