Table 3 Primer sequences and amplicon size for qPCR and dsRNA synthesis of the target genes hsp, shi, and iap.
Gene | Primer | Primer Sequence (5′-3′) | Amplicon (bp) |
|---|---|---|---|
hsp – heat shock protein | F-qRNA-HSP | ACACGCACACTCGTTCTCAC | 139 |
R-qRNA-HSP | TACGCGTACTCGCTGAAGAA | ||
F-dsRNA-HSP | TAATACGACTCACTATAGGGGTGCAG CAACTGGTCAAAGA | 351 | |
R-dsRNA-HSP | TAATACGACTCACTATAGGGGTCTTT GGTCATGGGACGTT | ||
shi – shibire | F-qRNA-SHI | AGTTCGCCGTTGATGAAATC | 86 |
R-qRNA-SHI | TCGAGCAGGGCTTTATGTCT | ||
F-dsRNA-SHI | TAATACGACTCACTATAGGGTAGATC GGTGTCAGTTCCCC | 379 | |
R-dsRNA-SHI | TAATACGACTCACTATAGGGGCGAGC GCGTTTTCTATTAC | ||
iap – inhibitor of apoptosis | F-qRNA-IAP | TTTCGTTTGATGCTCGACTG | 109 |
R-qRNA-IAP | TCTTCGCCTGTCCTGTCTTT | ||
F-dsRNA-IAP | TAATACGACTCACTATAGGGGTCCCGC TCATCCAGATAAA | 370 | |
R-dsRNA-IAP | TAATACGACTCACTATAGGGTTTTGCCT CTTTCGCACTTT |
