Figure 2

Fibre segments from human vastus lateralis muscle identified for myosin heavy chain (MHC) isoforms by dot blotting and Western blotting (A) 1/10 of each fibre segment was dot blotted (positions A1-10, B1-10), along with control muscle samples (+lane). Based on MHC isoform expression, fibre segments were characterised as type I (red circles), IIx (blue circles), or IIa (no circle). There was one dot/sample that remained negative for all three probes (green circles). (B) Fibre type of fibre segments from positions A1-10 and B1-10, was also determined by Western blotting using 1/5 of each fibre segment sample. The 170 kDa molecular marker can be seen for each blot (lanes labelled M). A single Stain Free gel is shown as a representation of the gels, and is indicative of the total protein loaded. Lanes are numbered according to the positions on the dot blots in (A) and below are indicated the assigned fibre type. Thirteen type IIa (black text), two type I (red text), and four type IIx fibre segments (blue text) were positively identified. Fibre type of the fibre segment at position B10 could not be determined using dot blotting, but was characterised as a type IIx fibre using Western blotting (green text).