Table 2 The 95% confidence interval widths for normalised protein content estimates derived from a simulated “pooling” of different numbers of type I and type IIa human skeletal muscle fibre segments.
From: A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres
Protein | Fibre type | Mean, a.u.a | 95% Confidence interval width, a.u. (% of mean)a | Break point, a.u. (SE)a | Slope, a.u. (SE)a,b | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Number of fibres pooled | |||||||||||||
1 | 5 | 10 | 15 | 20 | 25 | 30 | 35 | 40 | |||||
SERCA2A | I | 4.97 | 4.12 (83) | 2.25 (45) | 1.56 (31) | 1.20 (24) | 1.12 (23) | 1.03 (21) | 0.86 (17) | 0.83 (17) | 0.82 (17) | 6.6 (0.30) | −0.028 (0.002) |
SERCA1 | IIa | 0.94 | 0.72 (76) | 0.45 (48) | 0.29 (31) | 0.24 (26) | 0.21 (22) | 0.19 (20) | 0.18 (19) | 0.17 (18) | 0.15 (16) | 8.5 (0.33) | −0.005 (0.0004) |
AMPKβ2 | I | 1.08 | 0.91 (85) | 0.42 (39) | 0.28 (26) | 0.23 (21) | 0.20 (19) | 0.19 (17) | 0.17 (16) | 0.16 (14) | 0.14 (13) | 4.6 (0.24) | −0.006 (0.0005) |
IIa | 0.88 | 0.53 (60) | 0.21 (24) | 0.15 (17) | 0.13 (14) | 0.10 (12) | 0.10 (11) | 0.08 (9) | 0.08 (10) | 0.08 (9) | 3.5 (0.20) | −0.003 (0.0003) | |
COXIV | I | 1.88 | 1.87 (94) | 0.84 (43) | 0.60 (30) | 0.48 (24) | 0.42 (21) | 0.36 (18) | 0.34 (17) | 0.31 (16) | 0.29 (15) | 4.4 (0.24) | −0.012 (0.001) |
IIa | 1.15 | 1.30 (113) | 0.51 (44) | 0.35 (30) | 0.30 (26) | 0.25 (22) | 0.22 (19) | 0.22 (19) | 0.18 (16) | 0.18 (15) | 3.4 (0.21) | −0.008 (0.0009) | |
