Figure 2
From: Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure
Principal component (PCA) and clustering analyses of the plasma lipidomic datasets before and after treatments. All plots are based on concentrations of all quantified lipid species in all plasma samples before and after short-term (3 days) prednisolone (dogs P1-P8) or long-term (25 weeks) tetracosactide (dogs T1-T6) treatments. Female dogs have underlined IDs. (a,b) Heatmaps showing the full datasets with abundance from all quantified lipid species in the plasma samples before (empty bar) and after (filled bar) treatment with short-term prednisolone (A, blue) or long-term tetracosactide (B, red). Colour scale represents normalized and centered lipid concentrations per lipid species (in rows). Dog IDs in grey font indicate before, and blue and red, after prednisolone and tetracosactide treatment, respectively. (c,d) PCA showing the specific effects of long-term tetracosactide (red squares) treatment in principal components 1 (PC 1) and of short-term prednisolone treatment (blue triangles) in principal components 3 (PC 3). Samples taken before treatment are indicated with empty symbols and samples after treatment with filled symbols. (e,f) PCA of log2-fold changes before and after short-term prednisolone (dogs P1-P8, blue triangles) and long-term tetracosactide (dogs T1-T6; red squares) treatments, respectively. PC 1 vs. PC 2 highlights distinct plasma lipidome changes between the two treatments (e), whereas PC 3 vs. PC 4 indicates sex-specific effects of the treatments on the plasma lipidome, separating female and male dogs into two clusters ((e); purple and grey ellipses, respectively).
