Figure 3
From: Distinct Phenotypes Induced by Three Degrees of Transverse Aortic Constriction in Mice
Echocardiography shows graded reduction in cardiac function after 25G, 26G and 27G TAC. (a) Left ventricular ejection fraction, (b) fractional shortening and (c) representative images of M-Mode echocardiography after 28 days show systolic dysfunction. (d) Representative images of longitudinal LV strain vector velocities during systole and diastole for sham and 27G TAC mice. The velocity, direction and synchronicity of tissue movement is indicated by the green lines. (e) Echocardiography-derived regional LV longitudinal strain rate (LSR) and (f) radial SR (RSR) at 28 days after TAC. Diastolic function determined from the apical 4-chamber view, showing (g) Isovolumic relaxation time (IVRT) normalized to total ejection time, (h) mitral valve E wave deceleration time and (i) left atrial area normalized to body weight. (j) Representative images of left atrial areas (yellow dotted line) are from 28 days after TAC. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. sham, †P < 0.05 and †††P < 0.001 vs. 25G and ‡P < 0.001 vs. 26G TAC by 2-way ANOVA with repeated measures and Bonferroni’s post-test or by simple 2-way ANOVA (e–f), with Dunnett’s post-test. Coloured symbols indicate *P < 0.05 vs. sham and †P < 0.05 vs. 25G TAC at the time point indicated for the corresponding colour. n = 10–12.
